Custom Stable Cell Lines

Dr. Frank Weise
dep. Head of Department Pharma & Biotech
Group Leader Cell- & Molecular Biology

+49 7121 51530-56

E-Mail

We provide you with customized cell lines expressing your target gene of choice, to support target and biomarker screening in industry and academia. So far, we have generated:

• 250 cDNA expression vectors,
• 850 stable recombinant cell lines for the expression of
• 200 targets, using
• 150 maternal cell lines or primary cells.
• Numerous CRISPR-edited cell lines.

Target validation

• Cloning your gene of interest into a suitable expression vector
• Selection of promoters of different strengths
• Lentiviral transduction of a cell line / primary cell type of your choice
• Selection of stable recombinant cell lines (polyclonal or monoclonal), validated by gene expression analysis at the mRNA / protein level
• Characterisation with respect to target protein localisation by immunofluorescence microscopy or FACS analysis
• Full range of functional characterisations (e.g. cell proliferation, induction of apoptosis, metabolism; please inquire for further assays)

High-content analysis of cellular phenotypes

• Combine the Chromobody® technology with your gene of interest!
• Double expression of your target gene and of an indicative Chromobody® in a single cell line
• High-content analysis for monitoring cell cycle progression, nuclear morphology, cytoskeletal integrity