Our Publications
Protein profiling
DigiWest
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Bockmayr T, et. al. Multiclass cancer classification in fresh frozen and formalin-fixed paraffin-embedded tissue by DigiWest multiplex protein analysis. Laboratory Investigation 2020.
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Hendriks F. G. D, et. al. Clinically relevant Cytochrome P450 3A4 induction mechanisms and drug screening in three-dimensional spheroid cultures of primary human hepatocytes. Clinical Pharmacology & Therapeutics 2020.
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Jevtic M, et. al. Impact of intercellular crosstalk between epidermal keratinocytes and dermal fibroblasts on skin homeostasis. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research 2020
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Blosser W, et al. A pan-cancer transcriptome analysis identifies replication fork and innate immunity genes as modifiers of response to the CHK1 inhibitor prexasertib. Oncotarget 2020
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Treindl, F. "Array-based Western-blotting reveals spatial differences in hepatic signaling and metabolism following CAR activation." Archives of Toxicology, 2020.
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Inder, S., et al. "Multiplex profiling identifies clinically relevant signaling proteins in an isogenic prostate cancer model of radioresistance." Scientific Reports 9.1 2019.
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Naskou, Johanna, et al. "EZH2 loss drives resistance to carboplatin and paclitaxel in serous ovarian cancers expressing ATM." Molecular Cancer Research 2019.
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Zhan T, et al. MEK inhibitors activate Wnt signaling and induce stem cell plasticity in colorectal cancer. Nature Communications 2019
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Laner-Plamberger S, et al. H. differentially impacts gene expression of stromal cells from various tissues. Scientific Reports 2019
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Schumacher D, et al. Heterogeneous pathway activation and drug response modelled in colorectal-tumor-derived 3d cultures. PLoS Genetics 2019
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Hoerst K, et al. Regenerative potential of adipocytes in hypertrophic scars is mediated by myofibroblast reprogramming. J Mol Med 2019
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Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F. A high-throughput pipeline for validation of antibodies. Nature Methods 2018 | Contact an author.
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Lomnytska M, Pinto R, Becker S, Engström U, Gustafsson S, Björklund C, Templin M, Bergstrand J, Xu L, Widengren J, Epstein E, Franzen B, Auer G. Platelet protein biomarker panel for ovarian cancer diagnosis. Biomarker Research 2018 | Contact an author.
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De Boussac H, Gondeau C, Briolotti P, Duret C, Treindl F, Roemer M, Fabre JM, Herrero A, Ramos J, Maurel P, Templin M, Gerbal-Chaloin S, Daujat-Chavanieu M. Epidermal Growth Factor represses Constitutive Androstane Receptor expression in primary hepatocytes and favors regulation by Pregnane X Receptor. Drug Metab Dispos 2017 | Contact an author.
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Kissel M, Berndt S, Fiebig L, Kling S, Ji Q, Gu Q, Lang T Hafner FT, Teufel M, Zopf D. Antitumor effects of [...] in preclinical models of hepatocellular carcinoma. Oncotarget 2017.
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Menck K, Sönmezer C, Worst TS, Schulz M, Dihazi GH, Streit F, Erdmann G, Kling S, Boutros M, Binder C, Gross JC. Neutral sphingomyelinases control extracellular vesicles budding from the plasma membrane. J Extracell Vesicles 2017 | Contact an author.
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Augustin I, Dewi DL, Hundshammer J, Erdmann G, Kerr G, Boutros M. Autocrine Wnt regulates the survival and genomic stability of embryonic stem cells. Science Signaling 2017 | Contact an author.
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Treindl F, Ruprecht B, Beiter Y, Schultz S, Döttinger A, Staebler A, Joos TO, Kling S, Poetz O, Fehm T, Neubauer H, Kuster B, Templin MF. A bead-based western for high-throughput cellular signal transduction analyses. Nature Communications 2016 | Contact an author.
- Schultze-Rhonhof L, Marzi J, Carvajal Berrio DA, Holl M, Braun T, Schäfer-Ruoff F, Andress J, Bachmann C, Templin M, Brucker SY, Schenke-Layland K, Weiss M. Human tissue-resident peritoneal macrophages reveal resistance towards oxidative cell stress induced by non-invasive physical plasma. Front Immunol. 2024 Mar 5;15:1357340. doi: 10.3389/fimmu.2024.1357340. PMID: 38504975; PMCID: PMC10949891.
- Walter B, Hirsch S, Kuhlburger L, Stahl A, Schnabel L, Wisser S, Haeusser LA, Tsiami F, Plöger S, Aghaallaei N, Dick AM, Skokowa J, Schmees C, Templin M, Schenke-Layland K, Tatagiba M, Nahnsen S, Merk DJ, Tabatabai G. Functionally-instructed modifiers of response to ATR inhibition in experimental glioma. J Exp Clin Cancer Res. 2024 Mar 12;43(1):77. doi: 10.1186/s13046-024-02995-z. PMID: 38475864; PMCID: PMC10935927
- Heider J, Stahl A, Sperlich D, Hartmann SM, Vogel S, Breitmeyer R, Templin M, Volkmer H. Defined co-cultures of glutamatergic and GABAergic neurons with a mutation in DISC1 reveal aberrant phenotypes in GABAergic neurons. BMC Neurosci. 2024 Mar 4;25(1):12. doi: 10.1186/s12868-024-00858-z. PMID: 38438989; PMCID: PMC10910844.
- Warin J, Vedrenne N, Tam V, Zhu M, Yin D, Lin X, Guidoux-D'halluin B, Humeau A, Roseiro L, Paillat L, Chédeville C, Chariau C, Riemers F, Templin M, Guicheux J, Tryfonidou MA, Ho JWK, David L, Chan D, Camus A. In vitroand in vivo models define a molecular signature reference for human embryonic notochordal cells. iScience. 2024 Jan 26;27(2):109018. doi: 10.1016/j.isci.2024.109018. PMID: 38357665; PMCID: PMC10865399.
- Spahn S, Kleinhenz F, Shevchenko E, Stahl A, Rasen Y, Geisler C, Ruhm K, Klaumuenzer M, Kronenberger T, Laufer SA, Sundberg-Malek H, Bui KC, Horger M, Biskup S, Schulze-Osthoff K, Templin M, Malek NP, Poso A, Bitzer M. The molecular interaction pattern of lenvatinib enables inhibition of wild-type or kinase-mutated FGFR2-driven cholangiocarcinoma. Nat Commun. 2024 Feb 12;15(1):1287. doi: 10.1038/s41467-024-45247-6. PMID: 38346946; PMCID: PMC10861557.
- Anderle N, Schäfer-Ruoff F, Staebler A, Kersten N, Koch A, Önder C, Keller AL, Liebscher S, Hartkopf A, Hahn M, Templin M, Brucker SY, Schenke-Layland K, Schmees C. Breast cancer patient-derived microtumors resemble tumor heterogeneity and enable protein-based stratification and functional validation of individualized drug treatment. J Exp Clin Cancer Res. 2023 Aug 18;42(1):210. doi: 10.1186/s13046-023-02782-2. PMID: 37596623; PMCID: PMC10436441.
- van Maanen JC, Bach FC, Braun TS, Giovanazzi A, van Balkom BWM, Templin M, Wauben MHM, Tryfonidou MA. A Combined Western and Bead-Based Multiplex Platform to Characterize Extracellular Vesicles. Tissue Eng Part C Methods. 2023 Nov;29(11):493-504. doi: 10.1089/ten.TEC.2023.0056. Epub 2023 Aug 22. PMID: 37470213; PMCID: PMC10654656.
- Wuerger LTD, Kudiabor F, Alarcan J, Templin M, Poetz O, Sieg H, Braeuning A. Okadaic Acid Activates JAK/STAT Signaling to Affect Xenobiotic Metabolism in HepaRG Cells. Cells. 2023 Feb 28;12(5):770. doi: 10.3390/cells12050770. PMID: 36899906; PMCID: PMC10000888.
- Oeller M, Jaksch-Bogensperger H, Templin M, Gehwolf R, Rohde E, Schallmoser K, Laner-Plamberger S. Transcription Factors STAT3 and MYC Are Key Players of Human Platelet Lysate-Induced Cell Proliferation. Int J Mol Sci. 2022 Dec 13;23(24):15782. doi: 10.3390/ijms232415782. PMID: 36555426; PMCID: PMC9781157.
- Scerri J, Scerri C, Schäfer-Ruoff F, Fink S, Templin M, Grech G. PKC-mediated phosphorylation and activation of the MEK/ERK pathway as a mechanism of acquired trastuzumab resistance in HER2-positive breast cancer. Front Endocrinol (Lausanne). 2022 Oct 18;13:1010092. doi: 10.3389/fendo.2022.1010092. PMID: 36329884; PMCID: PMC9623415.
- Ruoff F, Kersten N, Anderle N, Jerbi S, Stahl A, Koch A, Staebler A, Hartkopf A, Brucker SY, Hahn M, Schenke-Layland K, Schmees C, Templin MF. Protein Profiling of Breast Carcinomas Reveals Expression of Immune-Suppressive Factors and Signatures Relevant for Patient Outcome. Cancers (Basel). 2022 Sep 19;14(18):4542. doi: 10.3390/cancers14184542. PMID: 36139700; PMCID: PMC9496820.
- Cumin C, Huang YL, Rossdam C, Ruoff F, Céspedes SP, Liang CY, Lombardo FC, Coelho R, Rimmer N, Konantz M, López MN, Alam S, Schmidt A, Calabrese D, Fedier A, Vlajnic T, von Itzstein M, Templin M, Buettner FFR, Everest-Dass A, Heinzelmann-Schwarz V, Jacob F. Glycosphingolipids are mediators of cancer plasticity through independent signaling pathways. Cell Rep. 2022 Aug 16;40(7):111181. doi: 10.1016/j.celrep.2022.111181. PMID: 35977490.
- Pfohl U, Loskutov J, Bashir S, Kühn R, Herter P, Templin M, Mamlouk S, Belanov S, Linnebacher M, Bürtin F, Vetter M, Reinhard C, Wedeken L, Regenbrecht CRA. A RAS-Independent Biomarker Panel to Reliably Predict Response to MEK Inhibition in Colorectal Cancer. Cancers (Basel). 2022 Jul 1;14(13):3252. doi: 10.3390/cancers14133252. PMID: 35805024; PMCID: PMC9265111.
RPPA
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Asperger H, Stamm N, Gierke B, Pawlak M, Hofmann U, Zanger U M, Marton A, Katona R L, Buhala A, Vizler C, Cieslik J P, Ruckhäberle E, Niederacher D, Fehm T, Neubauer H, Ludescher M. Progesterone receptor membrane component 1 regulates lipid homeostasis and drives oncogenic signaling resulting in breast cancer progression. Breast Cancer Research: BCR 2020 | Contact an author.
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Thejer B M, Adhikary P P, Kaur A, Teakel S L, Van Oosterum A, Seth I, Pajic M, Hannan K M, Pavy M, Poh P, Jazayeri J A, Zaw T, Pascovici D, Ludescher M, Pawlak M, Cassano J C, Turnbull L, Jazayeri M, James A C, Coorey C P, … Cahill M A. PGRMC1 phosphorylation affects cell shape, motility, glycolysis, mitochondrial form and function, and tumor growth. BMC Molecular and Cell Biology 2020 | Contact an author.
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Thejer B M, Adhikary P P, Teakel S L, Fang J, Weston P A, Gurusinghe S, Anwer A G, Gosnell M, Jazayeri J A, Ludescher M, Gray L A, Pawlak M, Wallace R H, Pant S D, Wong M, Fischer T, New E J, Fehm T N, Neubauer H, Goldys E M, Cahill M A. PGRMC1 effects on metabolism, genomic mutation and CpG methylation imply crucial roles in animal biology and disease. BMC Molecular and Cell Biology 2020 | Contact an author.
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Kresbach G M, Pawlak M. High Precision RPPA: Concept, Features, and Application Performance of the Integrated Zeptosens Platform. Advances in Experimental Medicine and Biology 2019 | Contact an author.
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Pawlak M, Carragher NO. Reverse Phase Protein Arrays Elucidate Mechanisms-of-action and Phenotypic Response in 2D and 3D models. Drug Discov Today Technol 2017 | Contact an author.
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Bader S, Zajac M, Friess T, Ruge E, Rieder N, Gierke B, Heubach Y, Thomas M, Pawlak M. Evaluation of Protein Profiles From Treated Xenograft Tumor Models Identifies an Antibody Panel for FFPE Tissue Analysis by RPPA. Mol Cell Proteomics 2015 | Contact an author.
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Tegnebratt T, Ruge E, Bader S, Ishii N, Aida S, Yoshimura Y, Ooi CH, Lu L, Mitsios N, Meresse V, Mulder J, Pawlak M, Venturi M, Tessier J, Stone-Elander S. Evaluation of Efficacy of a New MEK Inhibitor, RO4987655, in Human Tumor Xenografts by [(18)F] FDG-PET Imaging Combined with Proteomic Approaches. EJNMMI Research 2014 | Contact an author.
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Akbani R, Becker KF, Carragher N, Goldstein T, de Koning L, Korf U, Liotta L, Mills GB, Nishizuka SS, Pawlak M, Petricoin EF 3rd, Pollard HB, Serrels B, Zhu J. Realizing the Promise of RPPA for Clinical, Translational, and Basic Research: a Workshop Report: the RPPA Society. Mol Cell Proteomics 2014 | Contact an author.
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Assadi M, Lamerz J, Jarutat T, Farfsing A, Paul H, Gierke B, Breitinger E, Templin MF, Essioux L, Arbogast S, Venturi M, Pawlak M, Langen H, Schindler T. Multiple Protein Analysis of FFPE Tissue Samples with RPPA. Mol Cell Proteomics 2013 | Contact an author.
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Pirnia F, Pawlak M, Thallinger GG, Gierke B, Templin MF, Kappeler A, Betticher DC, Gloor B, Borner MM. Novel Functional Profiling Approach Combining RPPA and Human 3-D ex vivo Tissue Cultures: Expression of Apoptosis-related Proteins in Human Colon Cancer. Proteomics 2009 | Contact an author.
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Saturno G, Pesenti M, Cavazzoli C, Rossi A, Giusti AM, Gierke B, Pawlak M, Venturi M. Expression of Serine/Threonine Protein-kinases and Related Factors in Normal Monkey and Human Retinas: the Mechanistic Understanding of a CDK2 Inhibitor Induced Retinal Toxicity. Toxicologic Pathology 2007 | Contact an author.
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Pawlak M, Schick E, Bopp MA, Schneider MJ, Oroszlan P, Ehrat M. Zeptosens Protein Microarrays: A Novel High-Performance Microarray Platform for Low Abundance Protein Analysis. Proteomics 2002 | Contact an author.
Luminex
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Rachel J. Church, Gerd A. Kullak-Ublick, Jiri Aubrecht, Herbert L. Bonkovsky, Naga Chalasani, Robert J. Fontana, Jens Goepfert, Frances Hackman, Nicholas M. P. King, Simon Kirby, Patrick Kirby, Sif Ormarsdottir, Shelli J. Schomaker, Francis Wolenski, Nadir Arber, Michael Merz, John-Michael Sauer, Raul J. Andrade, Florian van Bömmel, Thierry Poynard, Paul B. Watkins. Candidate biomarkers for the diagnosis and prognosis of drug-induced liver injury: an international collaborative effort. Hepatology 2018.
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Filomena, A., Göpfert, J.C., Duffy, D., Pol, S., Abdel-Hamid, M., Esmat, G., Fontanet, A., Albert, M.L., Joos, T.O. and Schneiderhan-Marra, N. Study of the humoral immune response towards HCV genotype 4 using a bead-based multiplex serological assay. High Throughput 2017 | Contact an author.
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Endt, K., Goepfert, J.C., Omlin, A., Athanasiou, A., Tennstedt, P., Guenther, A., Rainisio, M., Engeler, D.S., Steuber, T., Gillessen, S., Joos, T.O. and Schiess, R. Development and clinical testing of individual immunoassays for the quantification of serum glycoproteins to diagnose prostate cancer. PLoS One 2017 | Contact an author.
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Walz, J. M., Boehringer, D., Deissler, H. L., Faerber, L., Goepfert, J. C., Heiduschka, P., Kleeberger, S. M., Klettner, A., Krohne, T. U., Schneiderhan-Marra, N., Ziemssen, F. and Stahl, A. Pre-Analytical Parameters Affecting Vascular Endothelial Growth Factor Measurement in Plasma: Identifying Confounders. PLoS One 2016 | Contact an author.
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Mikus, M., Drobin, K., Gry, M., Bachmann, J., Lindberg, J., Yimer, G., Aklillu, E., Makonnen, E., Aderaye, G., Roach, J., Fier, I., Kampf, C., Göpfert, J., Perazzo, H., Poynard, T., Stephens, C., Andrade, P., Lucena, M., Arber, N., Uhlén, M., Watkins, P., Schwenk, J., Nilsson, P. and Schuppe-Koistinen, I. Elevated levels of circulating CDH5 and FABP1 in association with human drug-induced liver injury. Liver International 2016 | Contact an author.
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Filomena, A., Göpfert, J., Duffy, D., Albert, M., Joos, T. and Schneiderhan‐Marra. Analysing the human serological response for HCV and eight other pathogens with a multiplexed antigen array. Journal of Viral Hepatitis 2013 | Contact an author.
Simoa
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Viviana A. Carcamo Yañez, Jens C. Göpfert, Markus Otto, Hayrettin Tumani, Andreas Peter, and Thomas O. Joos. Development and Validation of an Ultrasensitive Procalcitonin Sandwich Immunoassay. High Throughput 2017 | Contact an author.
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Müller, S., Preische, O., Göpfert, J.C., Carcamo Yañez, V.A., Joos, T.O., Boecker, H., Düzel, E., Falkai, P., Priller, J., Buerger, K., Catak, C., Janowitz, D., Heneka, M.T., Brosseron, F., Nestor, P., Peters, O., Menne, F. Schipke, C.G., Schneider, A., Spottke, A., Fließbach, K., Kilimann, I., Teipel, S., Wagner, M., Wiltfang, J., Jessen, F. and Laske, C. Plasma levels in subjective cognitive decline: Results from the DELCODE study. Scientific Reports 2017 | Contact an author.
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Schelle, J., Haesler, L., Goepfert, J. C., Joos, T. O., Vanderstichele, H., Stoops, E., Mandelkow, E. M., Neumann, U., Shimshek, D. R., Staufenbiel, M., Jucker, M. and Kaeser, S. A. Prevention of tau increase in cerebrospinal fluid of APP transgenic mice suggests downstream effect of BACE1 inhibition. Alzheimers Dement 2016 | Contact an author.
Custom cell services
Chromobody cell lines
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Keller BM, Maier J, Secker KA, Egetemaier SM, Parfyonova Y, Rothbauer U, Traenkle B. Chromobodies to quantify changes of endogenous protein concentration in living cells. Mol Cell Proteomics 2018 | Contact an author.
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Traenkle B, Rothbauer U. Under the Microscope: Single-Domain Antibodies for Live-Cell Imaging and Super-Resolution Microscopy. Front Immunol. 2017 | Contact an author.
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Maier J, Traenkle B, Rothbauer U. Visualising epithelial–mesenchymal transition (EMT) using the Chromobody Technology. Cancer Research 2016 | Contact an author.
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Schorpp K, Rothenaigner I, Maier J, Traenkle B, Jung M, Rothbauer U, Hadian K. A Multiplexed High-Content Screening Approach Using the Chromobody Technology to Identify Cell Cycle Modulators in Living Cells. J Biomol Screen 2016 | Contact an author.
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Buchfellner A., Yurlova L., Nueske S., Scholz A.M., Bogner J., Ruf B., Zolghadr K., Drexler S.E., Drexler G.A., Girst S., Greubel C., Reindl J., Siebenwirth C., Romer T., Friedl A.A., Rothbauer U. A new nanobody-based biosensor to study endogenous PARP1 in vitro and in live human cells. PLoS One 2016 | Contact an author.
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Maier J, Traenkle B, Rothbauer U. Real-time analysis of epithelial-mesenchymal transition using fluorescent nanobodies. Scientific Reports 2015 | Contact an author.
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Panza P, Maier J, Schmees C, Rothbauer U, Söllner C. Live imaging of endogenous protein dynamics in zebrafish using chromobodies. Development 2015 | Contact an author.
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Traenkle B, Emele F, Anton R, Poetz O, Haeussler RS, Maier J, Kaiser PD, Scholz AM, Nueske S, Buchfellner A, Romer T, Rothbauer U. Monitoring interactions and dynamics of endogenous beta-catenin with intracellular nanobodies in living cells. Mol Cell Proteomics 2015 | Contact an author.
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Kaiser PD, Maier J, Traenkle B, Emele F, Rothbauer U. Recent progress in generating intracellular functional antibody fragments to target and trace cellular components in living cells. Biochim Biophys Acta 2014 | Contact an author.
Electrophysiology
Patch clamp
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Kramer, J. et. al. Cross-site and cross-platform variability of automated patch clamp assessments of drug effects on human cardiac currents in recombinant cells. Scientific Reports 2020 | Contact an author.
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Ridder, B et. al. A systematic strategy for estimating hERG block potency and its implications in a new cardiac safety paradigm. Toxicology and Applied Pharmacology 2020 | Contact an author.
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Danker T, Braun F, Silbernagl N, Guenther E. Catch and Patch: A Pipette-Based Approach for Automating Patch Clamp That Enables Cell Selection and Fast Compound Application. ASSAY and Drug Development Technologies 2016 | Contact an author.
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Brun J, Lutz KA, Neumayer KMH, Klein G, Seeger T, Uynuk-Ool T, Wörgötter K, Schmid S, Kraushaar U, Guenther E, Rolauffs B, Aicher WK, Hart ML. Smooth Muscle-Like Cells Generated from Human Mesenchymal Stromal Cells Display Marker Gene Expression and Electrophysiological Competence Comparable to Bladder Smooth Muscle Cells. PLOS ONE 2015 | Contact an author.
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Danker T. and Möller C. Early identification of hERG liability in drug discovery programs by automated patch clamp. Front Pharmacol. 2014 | Contact an author.
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Dettmann HM, Zhang Y, Wronna N, Kraushaar U, Guenther E, Mohr R, Neckel PH, Mack A, Fuchs J, Just L, Obermayr F. Isolation, Expansion and Transplantation of Postnatal Murine Progenitor Cells of the Enteric Nervous System. PLOS ONE 2014 | Contact an author.
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Weiser T, Wronna N, Kraushaar U. Effects of Butylscopolamine Bromide on Nicotinic Acetylcholine Receptors in Cultured Guinea pig Enteric Neurons. Neurogastroenterol Motil. 2012 | Contact an author.
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Radtke C, Sinis N, Sauter M, Jahn S, Kraushaar U, Guenther E, et al. TRPV Channel Expression in Human Skin and Possible Role in Thermally Induced Cell Death. J Burn Care Res. 2011 | Contact an author.
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Metzger M, Bareiss PM, Danker T, Wagner S, Hennenlotter J, Guenther E, et al. Expansion and Differentiation of Neural Progenitors Derived from the Human Adult Enteric Nervous System. Gastroenterology 2009 | Contact an author.
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Lipp P, Viero C, Kraushaar U, Ruppenthal S, Kaestner L. A primary Culture System for Sustained Expression of Calcium Sensors and Chronic Electrical Stimulation in Adult Rat Ventricular Myocytes. Biophys J. 2007 | Contact an author.
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Kraushaar U, Backus KH. Characterization of GABA(A) and Glycine Receptors in Neurons of the Developing Rat Inferior Colliculus. Pflugers Arch. 2002 | Contact an author.
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Hefft S, Kraushaar U, Geiger JRP, Jonas P. Presynaptic Short-term Depression is Maintained During Regulation of Transmitter Release at a GABAergic Synapse in Rat Hippocampus. J Physiol. 2002 | Contact an author.
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Kraushaar U, Jonas P. Efficacy and Stability of Quantal GABA Release at a Hippocampal Interneuron-principal Neuron Synapse. J Neurosci. 2000 | Contact an author.
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Jonas P, Haverkampf K, Kraushaar U, Geiger JRP. Glutamate- and GABA-mediated Synaptic Transmission in the Principal Neuron-interneuron Network of the Dentate gyrus. J Physiol-London 1998 | Contact an author.
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Berger T, Schwarz C, Kraushaar U, Monyer H. Dentate gyrus basket cell GABAA Receptors are Blocked by Zn2+ via Changes of their Desensitization Kinetics: an in situ Patch-clamp and Single-cell PCR Study. J Neurosci. 1998 | Contact an author.
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Götz T, Kraushaar U, Geiger J, Lubke J, Berger T, Jonas P. Functional Properties of AMPA and NMDA Receptors Expressed in Identified Types of Basal Ganglia Neurons. J Neurosci. 1997 | Contact an author.
Oocyte recording
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Orhan G, Bock M, Schepers D, Ilina E, Reichel S, Löffler H, Jezutkovic N, Weckhuysen S, Mandelstam S, Suls A, Danker T, Guenther E, Scheffer I, De Jonghe P, Lerche H, Maljevic S. Dominant-Negative Effects of KCNQ2 Mutations Are Associated with Epileptic Encephalopathy. Ann Neurol. 2014 | Contact an author.
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Beissner M, Dutertre S, Schemm R, Danker T, Sporning A, Grubmuller H. Efficient Binding of 4/7 α-conotoxins to Nicotinic α4β2 Receptors is Prevented by R185 and P195 in the α4 Subunit. Mol Pharmacol. 2012 | Contact an author.
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Pehl U, Leisgen C, Gampe K, Guenther E. Automated Higher-Throughput Compound Screening on Ion Channel Targets Based on the Xenopus Laevis Oocyte Expression System. Assay and Drug Development Tech. 2004.
Live cell imaging
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Shen N, Knopf A, Westendorf C, Kraushaar U, Riedl J, Bauer H, Pöschel S, Layland S.L, Holeiter M, Knolle S, Brauchle E, Nsair A, Hinderer S, Schenke-Layland K. Steps toward Maturation of Embryonic Stem Cell-Derived Cardiomyocytes by Defined Physical Signals. Stem Cell Reports 2017 | Contact an author.
- Viero C, Kraushaar U, Ruppenthal S, Kaestner L, Lipp P. A Primary Culture System for Ssustained Expression of a Calcium Sensor in Preserved Adult Rat Ventricular Myocytes. Cell Calcium 2008 | Contact an author.
MEA-based systems
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Kraushaar, Udo, and Elke Guenther. "Assay Procedures for Compound Testing of hiPSC-Derived Cardiomyocytes Using Multiwell Microelectrode Arrays." Cell-Based Assays Using iPSCs for Drug Development and Testing." Methods Mol. Biol. 2019 | Contact an Author.
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Blinova K, Dang Q, Millard D, Smith G, Pierson J, Guo L, Brock M, Lu H.R, Kraushaar U, ..., Stockbridge N, Strauss D.G, Gintant G. International Multisite Study of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes for Drug Proarrhythmic Potential Assessment. Cell Reports 2018 | Contact an author.
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Millard D, Dang Q, Shi H, Zhang X, Strock C, Kraushaar U, ..., Ross J, Pierson J, Gintant G. Cross-Site Reliability of Human Induced Pluripotent stem cell-derived Cardiomyocyte Based Safety Assays Using Microelectrode Arrays: Results from a Blinded CiPA Pilot Study. Toxicological Sciences | Contact an author.
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Mulder P, de Korte T, Dragicevic E, Kraushaar U, Printemps R, Vlaming MLH. Predicting Cardiac Safety using Human Induced Pluripotent Stem Cell-derived Cardiomyocytes combined with MEA Technology. J of Pharmacological and Toxicological Methods 2018 | Contact an author.
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Loser D, Danker T, Möller C, Niedworok A, Kraushaar U. Development of a MEA based Neurotoxicity Assay for Detecting the Seizurogenic Activity of Novel Drug Candidates. Frontiers in Cellular Neuroscience 2018 | Contact an author.
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Kraushaar U, Guenther E, Hess D. Addressing Functional Neurotoxicity Using the Microelectrode Array (MEA). Stem Cell-Derived Models in Toxicology 2017 | Contact an author.
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Rast G, Kraushaar U, Buckenmaier S, Ittrich C, Guth BD. Influence of field Potential Duration on Spontaneous Beating rate of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes: Implications for Data Analysis and Test System Selection. J of Pharmacological and Toxicological Methods 2016 | Contact an author.
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Schönecker S, Kraushaar U, Guenther E, Gerst F, Ullrich S, Häring H.U, Königsrainer A, Barthlen W, Drews G, und Krippeit-Drews P. Human Islets Exhibit Electrical Activity on Microelectrode Arrays (MEA). Experimental and Clinical Endocrinology and Diabetes 2015 | Contact an author.
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Schönecker S, Kraushaar U, et al., Long-Term Culture and Functionality of Pancreatic Islets Monitored Using Microelectrode Arrays. Integrative Biology 2014 | Contact an author.
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Gerwig R, Fuchsberger K, Schroeppel B, Link GS, Heusel G, Kraushaar U, Schuhmann W, Stett A, Stelzle M. PEDOT-CNT Composite Microelectrodes for Recording and Electrostimulation Applications: Fabrication, Morphology, and Electrical Properties. Front. Neuroeng. 2012 | Contact an author.
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Meyer T, Guenther E, Kraushaar U. Microelectrode Arrays in Cardiac Mapping. Cardiac Mapping 2012 | Contact an author.
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Kraushaar U, Meyer T, Hess D, Gepstein L, L Mummery C, R Braam S, et al. Cardiac Safety Pharmacology: From Human ether-a-gogo Related Ggene Channel Block Towards Induced Pluripotent Stem Cell-based Disease Models. Expert Opinion on Drug Safety 2011 | Contact an author.
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Pfeiffer T, Kraushaar U, et al. Rapid functional evaluation of beta-cells by extracellular recording of membrane potential oscillations with Microelectrode Arrays. E. Journal of Physiology 2011 | Contact an author.
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Mandenius C-F, Steel D, Noor F, Meyer T, Heinzle E, Asp J. Cardiotoxicity Testing Using Pluripotent Stem Cell-derived Human Cardiomyocytes and State-of-the-art Bioanalytics. J Appl Toxicol 2011 | Contact an author.
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Meyer T, Stuerz K, Guenther E, Edamura M, Kraushaar U. Cardiac Slices as a Predictive Tool for Arrhythmogenic Potential of Drugs and Chemicals. Expert Opin Drug Metab Toxicol 2010 | Contact an author.
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Meyer T, Kraushaar U, Guenther E. MEA High-resolution Electrophysiological Mapping of Cardiac cell, Tissue and Organ Preparations. Springer Berlin Heidelberg 2009 | Contact an author.