CRISPR Cell Lines
Based on our long-standing expertise in generating customized stable cell lines, we are now also employing the CRISPR/Cas9 genome editing approach to develop your CRISPR-edited cell models.
Full freedom to operate (FTO) is ensured through a set of license agreements (please see our agreement with ERS Genomics).
We offer
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Introduction of specifically site-directed double stand breaks applying the CRISPR/Cas9 technology
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Generation of target-defective / knockout cell lines
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Larger deletions by two double strand breaks
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Insertion of specific mutations or extensions into the DNA locus with ectopic repair templates
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Generation of synthetic human iPSC disease models
- CRISPR editing of primary T cells
Beyond the production of custom cell lines, we employ the CRISPR/Cas9 technology also as part of our target validation studies, analogous to RNAi mediated target silencing.
Immunofluorescence image of C-terminal hemagglutinin tagging of endogenous HSP60 in breast carcinoma (A) and HEK293 (B) cells.