Gene Modulation by shRNAs and siRNAs

We employ inter alia lentiviral vectors to down-regulate the gene expression of target genes via shRNA-mediated RNA interference (RNAi). Alternatively, we use commercially available siRNAs for cell-based RNAi gene silencing experiments.

We offer

  • Generation of lentivirus encoding target-specific shRNAs

  • Production of stable knock-down cell lines

  • Validation of target silencing by RT-qPCR, Western blot and/or immunofluorescence microscopy

  • RNAi studies with shRNAs or siRNAs, using a wide range of reader-based and fixed endpoint or live microscopy assays

 

A further expansion of possibilities is provided by the CRISPR/Cas9 technology, which we employ to abrogate target expression.

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